84-3648-80　［取扱停止］PTEN Tumor Suppressor Protein (MMAC1, Phosphatase and Tensin, TEP1) (AP) 100ul　P9182-02-AP

特徴

• PTEN (also know as MMAC1) was recently identified as a candidate tumor suppressor gene.
• Deletions or mutations within the PTEN gene have been observed in a variety of tumor cell lines and cancer cell types.
• Based on PTEN’s structural features, the protein was originally thought to be a protein tyrosine phosphatase.
• However, recent evidence indicates that PTEN is actually a lipid phosphatase rather that a protein tyrosine phosphatase.
• In fact, the target substrate for PTEN is phosphatidylinositol-3,4,5-triphosphate (Ptd-Ins(3,4,5)P3).
• Ptd-Ins(3,4,5)P3 is a critical second messenger involved in cell signaling and growth control.
• Ptd-Ins(3,4,5)P3 is produced by phosphorylation of Ptd-Ins(4,5)P2 by PI 3-kinase in response to stimulation by various ligands.
• By dephosphorylating Ptd-Ins(3,4,5)P3, PTEN functions to “turn off” the Ptd-Ins(3,4,5)P3 pathway and thereby suppress cellular proliferation.
• Applications:Suitable for use in ELISA, Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 0.1–1.0ug/mlWestern Blot: 1–3ug/ml
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.