MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
Transferrins are iron-binding blood plasma glycoproteins that control the level of free iron in biological fluids.
In humans, it is encoded by the TF gene.
In humans, transferrin consists of a polypeptide chain containing 679 amino acids.
The protein is composed of alpha helices and beta sheets to form two domains.
The N- and C- terminal sequences are represented by globular lobes and between the two lobes is an iron-binding site.
Transferrin is a glycoprotein that binds iron very tightly but reversibly.
Although iron bound to transferrin is less than 0.1% (4 mg) of the total body iron, it is the most important iron pool, with the highest rate of turnover (25 mg/24 h).
Transferrin has a molecular weight of around 80 kDa and contains 2 specific high-affinity Fe(III) binding sites.
The affinity of transferrin for Fe(III) is extremely high (1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality.
UniProt Number:P02787
Gene ID:TF
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry (Paraffin).
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™650 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with MaxLight™650.
Specificity：Recognizes human Transferrin. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：Oryza sativa-derived human Transferrin recombinant protein (Position: V20-P698).
