Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31.
Human FGF1 shares 96% amino acid sequence homology with both rat and mouse.
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family.
FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor.
It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
Additionally, Acidic fibroblast growth factor is derived from beta-endothelial cell growth factor (ECGFB) by posttranslational processing.
Alpha-ECGF is also derived from ECGFB in the same manner.
Applications:Suitable for use in FLISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilution:Immunohistochemistry (Paraffin): Boiling paraffin sections in 10mM citrate buffer, pH 6 for 20 min is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Handling:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes human FGF1.
Isotype:IgG
Calc Applications Abbrev:FLISA IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human FGF1, expressed in E. coli (Position: F16-D155). Species Sequence Homology: mouse and rat, 96%