ADIPOQ(Adipocyte-, C1q-, and Collagen Domain-Containing), also known as APM1, ADPN or ACDC, is a protein which in humans is encoded by the ADIPOQ gene.
Using FISH, Das et al.
(2001) mapped the mouse Acrp30 gene to chromosome 16 in a region showing homology of synteny with human 3q27.
By RNase protection and Western blot analysis, Schaffler et al.
(1999) showed that APM1 is expressed by differentiated adipocytes as a 33-kD protein that is also detectable in serum.
By sequence comparisons, they found links between APM1 and TNF family ligands as well as to cytokines expressed by T cells.
Adiponectin is a protein hormone that modulates a number of metabolic processes, including glucose regulation and fatty acid oxidation.
Adiponectin is exclusively secreted from adipose tissue (and also from the placenta in pregnancy) into the bloodstream and is very abundant in plasma relative to many hormones.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Antigen retrieval by boiling paraffin sections in 10mM citrate buffer, pH 6.0 for 20 minutes is required for the staining of paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Recognizes human ADIPOQ. Species Crossreactivity: mouse and rat
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：Synthetic peptide corresponding to aa224-244, LYADNDNDSTFTGFLLYHDTN from the C-terminus of human ADIPOQ, different from the related rat and mouse sequences by one amino acid.
