Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kD pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid".
It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins.
This gene is mapped to 1q23.2.
The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone.
This protein is also thought to control the degradation of chromatin.
It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Applications:Suitable for use in Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Western Blot: 0.1-0.5ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Lyophilized powder may be stored at -20°C.
Stable for 12 months after receipt at -20°C.
Reconstitute with sterile ddH2O.
Aliquot to avoid repeated freezing and thawing.
Store at -20°C.
Reconstituted product is stable for 12 months at -20°C.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Further dilutions can be made in assay buffer.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity：Recognizes rat Serum Amyloid P.
Isotype：IgG
Calc Applications Abbrev：IHC WB
Calc Crossreactivity：Rt
Immunogen：Recombinant protein corresponding to rat Serum Amyloid P expressed in E. coli. (Position: Q21-S228). Species sequence homology: human (72%) and mouse (80%)
