Fibroblast growth factor 1 (acidic), also known as FGF1/ECGF/HBGF1, is a human gene which is mapped to 5q31.
Human FGF1 shares 96% amino acid sequence homology with both rat and mouse.
The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family.
FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion.
This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor.
It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
Additionally, Acidic fibroblast growth factor is derived from beta-endothelial cell growth factor (ECGFB) by posttranslational processing.
Alpha-ECGF is also derived from ECGFB in the same manner.
Applications:Suitable for use in ELISA, Western Blot and Immunohistochemistry.
Other applications have not been tested.
Recommended Dilutions:Immunohistochemistry (Paraffin): Boiling paraffin sections in 10mM citrate buffer, pH 6 for 20 min is required for the staining of formalin/paraffin sections
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Affinity Purified
Purity:Purified by immunoaffinity chromatography.
Form:Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.
Specificity:Recognizes human FGF1.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Recombinant protein corresponding to human FGF1, expressed in E. coli (Position: F16-D155). Species Sequence Homology: mouse and rat, 96%