Cullin-3(Cul 3), is a protein that in humans is encoded by the CUL3 gene.
Kipreos et al.
(1996) identified a conserved gene family, designated cullins, with at least 5 members in nematodes, 6 in humans, and 3 in S.
cerevisiae.
Human CUL3 is an ortholog of nematode cul3.
Hartz (2012) mapped the CUL3 gene to chromosome 2q36.2 based on an alignment of the CUL3 sequence with the genomic sequence (GRCh37).
Sumara et al.
(2007) found that KLHL9, KLHL13, and CUL3 interacted directly in a 370-kD protein complex in HeLa cell lysates.
Rondou et al.
(2008) showed that interaction between KLHL12 and the CUL3 ubiquitin ligase complex directed ubiquitination of dopamine receptor D4 (DRD4).
KLHL12 interacted directly with CUL3 and with the polymorphic intracellular loop-3 of D4.
By immunoprecipitation analysis of HeLa cells and HCT116 human colon carcinoma cells expressing epitope-tagged proteins, Kigoshi et al.
(2011) showed that KLHL7 interacted with CUL3 and ROC1 (RBX1).
UniProt Number:Q13618
Gene ID:CUL3
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by immunoaffinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. Labeled with Alkaline Phosphatase (AP).
Specificity：Recognizes human Cullin3. Species Crossreactivity: rat. No crossreactivity with other proteins.
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Rt
Immunogen：A synthetic peptide corresponding to aa426-444, DVFERYYKQHLARRLLTNK in the middle region of human Cullin 3 (Q13618). Species Sequence Homology: mouse
