MaxLight™ 750 is a new Near IR stable dye conjugate comparable to DyLight™750, Alexa Fluor™700 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes.
CCL2 is structurally related to the CXC subfamily of cytokines.
Members of this subfamily are characterized by two cysteines separated by a single amino acid.
This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils.
It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis.
It binds to chemokine receptors CCR2 and CCR4.
Applications:Suitable for use in FLISA, Western Blot, Immunofluorescence, Flow Cytometry (Not Tested) and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1000Western Blot: 1:50-1:100Immunohistochemistry: 1:50-1:100Immunofluorescence: 1:10-1:50Flow Cytometry (Not Tested): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™750 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:100ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™750.
Specificity:Recognizes human CCL2.
Isotype:IgG
Calc Applications Abbrev:FLISA IF IHC WB
Calc Crossreactivity:Hu
Immunogen:KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa68-98 from the C-terminal region of human CCL2.