84-3607-82　［取扱停止］FEN1, ID (Flap Endonuclease 1, Flap Structure-specific Endonuclease 1, Maturation Factor 1, MF1, DNase IV, hFEN-1, RAD2) (MaxLight 650) 100ul　F0047-02A-ML650

特徴

• MaxLight™ 650 is a new Far-IR stable dye conjugate comparable to Alexa Fluor™647, DyLight™649, Cy5™ and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (655nm); Emission (676nm); Extinction Coefficient 250,000.
• FEN1 removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis.
• Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another.
• This protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication.
• DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene.
• Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
• Applications:Suitable for use in FLISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™650 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.