84-3605-85　［取扱停止］Zcchc11 (Terminal Uridylyltransferase 4, TUTase 4, Zinc Finger CCHC Domain-Containing Protein 11, Zcchc11, Kiaa0191, Tut4) (MaxLight 550) 100ul　302853-ML550

特徴

• MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
• Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs.
• Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor.
• Uridylated pre- let-7 miRNAs fail to be processed by Dicer and undergo degradation.
• Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency.
• Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B (By similarity).
• Also catalyzes the 3' uridylation of miR- 26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression.
• May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.
• Does not play a role in replication-dependent histone mRNA degradation (By similarity).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™550 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.