MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
Required both for the formation and autophagic degradation of polyubiquitin-containing bodies, called ALIS (aggresome-like induced structures).
Links ALIS to the autophagic machinery via direct interaction with MAP1 LC3 family members.
May regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1.
May play a role in titin/TTN downstream signaling in muscle cells.
May regulate signaling cascades through ubiquitination.
May be involved in cell differentiation, apoptosis, immune response and regulation of K(+) channels.
Adapter that mediates the interaction between TRAF6 and CYLD.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes Sqstm1. Species Crossreactivity: human, mouse
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH conjugated synthetic peptide corresponding to aa346-379 at the human region of human Phospho-Sqstm1(S351). Species sequence homology: rat
