MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain.
This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase.
It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3.
This protein may play a role in the repression of neuronal genes.
Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.
It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and FLISA.
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™550 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™550.
Specificity：Recognizes human LSD1.
Isotype：IgG
Calc Applications Abbrev：FLISA IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 819-852 in the C-terminal region of human LSD1, UniProt Accession #O60341. Species Sequence Homology: Mouse
