84-3587-80　［取扱停止］Isocitrate Dehydrogenase, CT (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Precursor], Isocitric dehydrogenase, NAD(+)-specific ICDH, IDH3A, IDH3) (MaxLight 550) 100ul　I8850-01E-ML550

特徴

• MaxLight™ 550 is a new Yellow-Green photostable dye conjugate comparable to Alexa Fluor™546, 555, DyLight™549 , Cy3™, TRITC and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (550nm); Emission (575nm); Extinction Coefficient 150,000.
• Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate.
• These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).
• Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic.
• NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle.
• Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.
• The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
• Applications:Suitable for use in FLISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™550 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.