MaxLight™ 490 is a new Blue-Green photostable dye conjugate comparable to DyLight™488, Alexa Fluor™488 and offers better labeling efficiency, brighter imaging and increased immunodetection.
ZWINT is clearly involved in kinetochore function although an exact role is not known.
It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores.
The protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase.
It has a uniform distribution in the cytoplasm of interphase cells.
Applications:Suitable for use in FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Flow Cytometry (Not Tested): 1:10-1:50Immunohistochemistry: 1:50-1:100Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher
Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™490 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™490.
Specificity：Recognizes human ZWINT. Species Crossreactivity: mouse.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：KLH-conjugated synthetic peptide between amino acids 66-96 in the center region of human ZWINT
