MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Catalytic subunit of a constitutively active serine/threonine-protein kinase complex that phosphorylates a large number of substrates containing acidic residues C-terminal to the phosphorylated serine or threonine.
Regulates numerous cellular processes, such as cell cycle progression, apoptosis and transcription, as well as viral infection.
May act as a regulatory node which integrates and coordinates numerous signals leading to an appropriate cellular response.
During mitosis, functions as a component of the p53/TP53-dependent spindle assembly checkpoint (SAC) that maintains cyclin-B-CDK1 activity and G2 arrest in response to spindle damage.
Also required for p53/TP53-mediated apoptosis, phosphorylating 'Ser-392' of p53/TP53 following UV irradiation.
Can also negatively regulate apoptosis.
Phosphorylates the caspases CASP9 and CASP2 and the apoptotic regulator NOL3.
Phosphorylation protects CASP9 from cleavage and activation by CASP8, and inhibits the dimerization of CASP2 and activation of CASP8.
Regulates transcription by direct phosphorylation of RNA polymerases I, II, III and IV.
Also phosphorylates and regulates numerous transcription factors including NF-kappa-B, STAT1, CREB1, IRF1, IRF2, ATF1, SRF, MAX, JUN, FOS, MYC and MYB.
Phosphorylates Hsp90 and its co-chaperones FKBP4 and CDC37, which is essential for chaperone function.
Regulates Wnt signaling by phosphorylating CTNNB1 and the transcription factor LEF1.
Acts as an ectokinase that phosphorylates several extracellular proteins.
Applications:Suitable for use in Western Blot.
Other applications not tested.
Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：mouse
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes R Csnk2a1. Species Crossreactivity: human, rat
Isotype：IgG
Calc Applications Abbrev：WB
Calc Crossreactivity：Hu Mo Rt
Immunogen：KLH conjugated synthetic peptide corresponding to aa28-50 at the N-terminal region of Rat Csnk2a1. Species sequence homology: bovine, chicken, mouse, rabbit, xenopus, C.Elegans, drosophila
