84-3558-52　［取扱停止］VDAC1, NT (Voltage-dependent Anion-selective Channel Protein 1, VDAC-1, hVDAC1, Outer Mitochondrial Membrane Protein Porin 1, Plasmalemmal Porin, Porin 31HL, Porin 31HM, VDAC) (MaxLight 405) 100ul　V2121-20D-ML405

特徴

• MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
• Absorbance (400nm); Emission (423nm); Extinction Coefficient 32,000.
• VDAC1 forms a channel through the mitochondrial outer membrane and also the plasma membrane.
• The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis.
• It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV.
• The open state has a weak anion selectivity whereas the closed state is cation-selective.
• The protein may participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis.
• Applications:Suitable for use in FLISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:FLISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: MaxLight™405 conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.