84-3554-93 ［取扱停止］F10, CT (Coagulation Factor X, Stuart Factor, Stuart-Prower Factor, Factor X Light Chain, Factor X Heavy Chain, Activated Factor Xa Heavy Chain) (MaxLight 405) 100ul F0019-40-ML405
84-3554-93 ［取扱停止］F10, CT (Coagulation Factor X, Stuart Factor, Stuart-Prower Factor, Factor X Light Chain, Factor X Heavy Chain, Activated Factor Xa Heavy Chain) (MaxLight 405) 100ul F0019-40-ML405
MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
F10 is the vitamin K-dependent coagulation factor X of the blood coagulation cascade.
This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR.
Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases.
The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway).
The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting.
Applications:Suitable for use in Immunohistochemistry, Flow Cytometry (Not Tested), FLISA and Western Blot.
Other applications not tested.
Recommended Dilution:Flow Cytometry (Not Tested): 1:10-1:50Immunohistochemistry (Paraffin): 1:50-1:100Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human F10.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 351-381 in the C-terminal region of human F10, UniProt Accession #P00742.
