MaxLight™ 405 is a new Violet photostable dye conjugate comparable to Alexa Fluor® 405, PacificBlue™, Brilliant™ Violet 421 and offers better labeling efficiency, brighter imaging and increased immunodetection.
Unlike most eukaryotic kinases, alpha kinases, such as ALPK1, recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
Epithelial cells maintain a polarized structure based on a selective sorting machinery for cargo traveling to the apical or the basolateral membrane domain at the trans-Golgi network exit.
Alpha-kinase 1 (ALPK1)is a compnent of raft-carrying apical vesicles, originally identified in vesicles ferrying raft-associated sucrase-isomaltase (SI).
It has been proposed that phosphorylation of myosin I by ALPK1 is essential to the apical trafficking of raft-associated SI.
Applications:Suitable for use in FLISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:FLISA: 1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: MaxLight™405 conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight™405.
Specificity：Recognizes human ALPK1.
Isotype：IgG
Calc Applications Abbrev：FLISA IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the C-terminal region of human ALPK1 (KLH).
