84-3544-86　［取扱停止］Ehmt2 (Histone-lysine N-Methyltransferase EHMT2, 211-, Euchromatic Histone-lysine N-Methyltransferase 2, HLA-B-Associated Transcript 8, Histone H3-K9 Methyltransferase 3, H3-K9-HMTase 3, Protein G9a, Ehmt2, Bat8, G9a, Ng36) (PE) 200ul　302411-PE

特徴

• Histone methyltransferase that specifically mono- and dimethylates 'Lys-9' of histone H3 (H3K9me1 and H3K9me2, respectively) in euchromatin.
• H3K9me represents a specific tag for epigenetic transcriptional repression by recruiting HP1 proteins to methylated histones.
• Also mediates monomethylation of 'Lys-56' of histone H3 (H3K56me1) in G1 phase, leading to promote interaction between histone H3 and PCNA and regulating DNA replication.
• Also weakly methylates 'Lys-27' of histone H3 (H3K27me).
• Also required for DNA methylation, the histone methyltransferase activity is not required for DNA methylation, suggesting that these 2 activities function independently.
• Probably targeted to histone H3 by different DNA-binding proteins like E2F6, MGA, MAX and/or DP1.
• May also methylate histone H1.
• In addition to the histone methyltransferase activity, also methylates non-histone proteins: mediates dimethylation of 'Lys- 373' of p53/TP53.
• Also methylates CDYL, WIZ, ACIN1, DNMT1, HDAC1, ERCC6, KLF12 and itself.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:2000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.