84-3544-82　［取扱停止］Eed (Polycomb Protein EED, Eed) (PE) 200ul　302406-PE

特徴

• Polycomb group (PcG) protein.
• Component of the PRC2/EED- EZH2 complex, which methylates 'Lys-9' and 'Lys-27' of histone H3, leading to transcriptional repression of the affected target gene.
• Also recognizes 'Lys-26' trimethylated histone H1 with the effect of inhibiting PRC2 complex methyltransferase activity on nucleosomal histone H3 'Lys-27', whereas H3 'Lys-27' recognition has the opposite effect, enabling the propagation of this repressive mark (By similarity).
• The PRC2/EED-EZH2 complex may also serve as a recruiting platform for DNA methyltransferases, thereby linking two epigenetic repression systems (By similarity).
• Genes repressed by the PRC2/EED-EZH2 complex include HOXA7, HOXB6 and HOXC8.
• Plays a role in X chromosome inactivation (XCI), in which one of the two X chromosomes in female mammals is transcriptionally silenced to equalize X-linked gene dosage with XY males.
• Required for stable maintenance of XCI in both embryonic and extraembryonic tissues.
• May prevent transcriptional activation of facultative heterochromatin during differentiation.
• Required for development of secondary trophoblast giant cells during placental development.
• May regulate hippocampal synaptic plasticity in the developing brain.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.