84-3544-74　［取扱停止］DPPA3 (Developmental Pluripotency-Associated Protein 3, Compaction-Associated Protein 1, Primordial Germ Cell Protein 7, Dppa3, Cap1p, Crg1, Pgc7) (PE) 200ul　302398-PE

特徴

• Primordial germ cell (PGCs)-specific protein involved in epigenetic chromatin reprogramming in the zygote following fertilization.
• In zygotes, DNA demethylation occurs selectively in the paternal pronucleus before the first cell division, while the adjacent maternal pronucleus and certain paternally-imprinted loci are protected from this process.
• Participates in protection of DNA methylation in the maternal pronucleus by preventing conversion of 5mC to 5hmC: specifically recognizes and binds histone H3 dimethylated at 'Lys-9' (H3K9me2) on maternal genome, and protects maternal genome from TET3-mediated conversion to 5hmC and subsequent DNA demethylation.
• Does not bind paternal chromatin, which is mainly packed into protamine and does not contain much H3K9me2 mark.
• Also protects imprinted loci that are marked with H3K9me2 in mature sperm from DNA demethylation in early embryogenesis.
• May be important for the totipotent/pluripotent states continuing through preimplantation development.
• Also involved in chromatin condensation in oocytogenesis.
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: PE conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.