Unlike most eukaryotic kinases, alpha kinases, such as ALPK1, recognize phosphorylation sites in which the surrounding peptides have an alpha-helical conformation.
Epithelial cells maintain a polarized structure based on a selective sorting machinery for cargo traveling to the apical or the basolateral membrane domain at the trans-Golgi network exit.
Alpha-kinase 1 (ALPK1)is a compnent of raft-carrying apical vesicles, originally identified in vesicles ferrying raft-associated sucrase-isomaltase (SI).
It has been proposed that phosphorylation of myosin I by ALPK1 is essential to the apical trafficking of raft-associated SI.
Applications:Suitable for use in ELISA and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity：Recognizes human ALPK1.
Isotype：IgG
Calc Applications Abbrev：E IHC
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide selected from the N-terminal region of human ALPK1 (KLH).
