KRT10 is a member of the type I (acidic) cytokeratin family, which belongs to the superfamily of intermediate filament (IF) proteins.
Keratins are heteropolymeric structural proteins which form the intermediate filament.
These filaments, along with actin microfilaments and microtubules, compose the cytoskeleton of epithelial cells.
Mutations in its gene are associated with epidermolytic hyperkeratosis.
Applications:Suitable for use in ELISA, Flow Cytometry (Not Tested), Western Blot and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Flow Cytometry (Not Tested): 1:10-1:50Western Blot: 1:50-1:100Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: PE conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Specificity:Recognizes human KRT10.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa325-354 from the center region of human KRT10, conjugated to KLH.