84-3530-18　［取扱停止］Zcchc11 (Terminal Uridylyltransferase 4, TUTase 4, Zinc Finger CCHC Domain-Containing Protein 11, Zcchc11, Kiaa0191, Tut4) (HRP) 200ul　302853-HRP

特徴

• Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs.
• Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor.
• Uridylated pre- let-7 miRNAs fail to be processed by Dicer and undergo degradation.
• Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency.
• Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B (By similarity).
• Also catalyzes the 3' uridylation of miR- 26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression.
• May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP.
• Does not play a role in replication-dependent histone mRNA degradation (By similarity).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.