PHLPP2 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1.
This protein regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes.
Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decrease cell proliferation.
It also controls the phosphorylation of AKT3.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry (Not Tested)
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity：Recognizes human PHLPP2
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu
Immunogen：PHLPP2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1301-1331 amino acids from the C-terminal region of human PHLPP2.
