84-3522-85　［取扱停止］HIF1A, NT (HIF1A, BHLHE78, MOP1, PASD8, Hypoxia-inducible factor 1-alpha, ARNT-interacting protein, Basic-helix-loop-helix-PAS protein MOP1, Class E basic helix-loop-helix protein 78, Member of PAS protein 1, PAS domain-containing protein 8) (HRP) 200ul　036551-HRP

特徴

• Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia.
• HIF1 is a heterodimer composed of an alpha subunit and a beta subunit.
• The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).
• This protein encodes the alpha subunit of HIF-1.
• Overexpression of a natural antisense transcript (aHIF) of this gene has been shown to be associated with nonpapillary renal carcinomas.
• Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-100Immunohistochemistry: 1:50-100Immunofluorescence: 1:10-50
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.