KIT is the human homolog of the proto-oncogene c-kit.
C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit.
KIT is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor).
Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism.
Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry (Not Tested), and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Immunohistochemistry: 1:10-1:50Flow Cytometry (Not Tested): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes human KIT.
Isotype:IgG
Calc Applications Abbrev:E
Calc Crossreactivity:Hu
Immunogen:Synthetic peptide selected from the N-term region of human KIT (KLH).