ETV4 is a transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.
Applications:Suitable for use in ELISA, Western Blot, Immunohistochemistry and Flow Cytometry (Not Tested).
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000Western Blot: 1:50-1:100Immunohistochemistry: 1:50- 1:100Flow Cytometry (Not Tested): 1:10- 1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
Specificity:Recognizes human ETV4. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:E IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa426-455 from the C-terminal region of human ETV4.