Receptor-activated non-selective cation channel involved in detection of sensations such as coolness, by being activated by cold temperature below 25 degrees Celsius.
Activated by icilin, eucalyptol, menthol, cold and modulation of intracellular pH.
Involved in menthol sensation.
Permeable for monovalent cations sodium, potassium, and cesium and divalent cation calcium.
Temperature sensing is tightly linked to voltage-dependent gating.
Activated upon depolarization, changes in temperature resulting in graded shifts of its voltage-dependent activation curves.
The chemical agonists menthol functions as a gating modifier, shifting activation curves towards physiological membrane potentials.
Temperature sensitivity arises from a tenfold difference in the activation energies associated with voltage-dependent opening and closing.
Applications:Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, ELISA
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity：Human
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 270-300 amino acids from the Central region of human TRPM8.
