84-3499-10 [取扱停止]Hypoxia Inducible Factor 1 alpha, CT (Hypoxia-inducible Factor 1-alpha, HIF1-alpha, HIF-1alpha, HIF-1-alpha, HIF1A, HIF1, ARNT-interacting Protein, Basic-helix-loop-helix-PAS Protein MOP1, Class E Basic Helix-loop-helix Protein 78, bHLHe78, Member of PAS Protein 1, MOP1, PAS Domain-containing Protein 8, PASD8) (FITC) 200ul H9804-03C-FITC
84-3499-10 [取扱停止]Hypoxia Inducible Factor 1 alpha, CT (Hypoxia-inducible Factor 1-alpha, HIF1-alpha, HIF-1alpha, HIF-1-alpha, HIF1A, HIF1, ARNT-interacting Protein, Basic-helix-loop-helix-PAS Protein MOP1, Class E Basic Helix-loop-helix Protein 78, bHLHe78, Member of PAS Protein 1, MOP1, PAS Domain-containing Protein 8, PASD8) (FITC) 200ul H9804-03C-FITC
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia.
HIF1 is a heterodimer composed of an alpha subunit and a beta subunit.
The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100-1:500Immunofluorescence: 1:10-1:50Immunohistochemistry (FFPE): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: FITC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein isothiocyanate (FITC).
Specificity:Recognizes human HIF1A at ~92kD. Species Crossreactivity: mouse
Isotype:IgG
Calc Applications Abbrev:IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa736-765 in the C-terminal region of human HIF1A, UniProt Accession #NP_001521; Q16665 Cellular Localization: Cytoplasm, Nucleus