84-3482-31　［取扱停止］SKP2, ID (S-phase Kinase-associated Protein 2, F-box Protein Skp2, Cyclin A/CDK2-associated Protein p45, p45skp2, F-box/LRR-repeat Protein 1, FBXL1) (Biotin) 200ul　S5440-51A-Biotin

特徴

• SKP2 is a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box.
• The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination.
• The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs.
• The protein encoded by this gene belongs to the Fbls class; in addition to an F-box, this protein contains 10 tandem leucine-rich repeats.
• This protein is an essential element of the cyclin A-CDK2 S-phase kinase.
• It specifically recognizes phosphorylated cyclin-dependent kinase inhibitor 1B (CDKN1B, also referred to as p27 or KIP1) predominantly in S phase and interacts with S-phase kinase-associated protein 1 (SKP1 or p19).
• In addition, this gene is established as a protooncogene causally involved in the pathogenesis of lymphomas.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.