PHLPP2 is a protein phosphatase that specifically mediates dephosphorylation of 'Ser-473' of AKT1.
This protein regulates the balance between cell survival and apoptosis through a cascade that primarily alters the function of transcription factors that regulate pro- and antiapoptotic genes.
Dephosphorylation of 'Ser-473' of AKT1 triggers apoptosis and decrease cell proliferation.
It also controls the phosphorylation of AKT3.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human PHLPP2.
Isotype：IgG
Calc Applications Abbrev：E WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa11-40 in the N-terminal region of human PHLPP2, UniProt Accession #NP_055835; Q6ZVD8
