Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes.
CCL2 is structurally related to the CXC subfamily of cytokines.
Members of this subfamily are characterized by two cysteines separated by a single amino acid.
This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils.
It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis.
It binds to chemokine receptors CCR2 and CCR4.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence, Flow Cytometry (Not Tested) and Immunohistochemistry.
Other applications not tested.
Recommended Dilution:ELISA: 1:1000Western Blot: 1:50-1:100Immunohistochemistry: 1:50-1:100Immunofluorescence: 1:10-1:50Flow Cytometry (Not Tested): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human CCL2.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide corresponding to a portion of amino acids within aa68-98 from the C-terminal region of human CCL2.
