Glucagon is actually a preproprotein that is cleaved into four distinct mature peptides.
One of these, glucagon, is a pancreatic hormone that counteracts the glucose-lowering action of insulin by stimulating glycogenolysis and gluconeogenesis.
Glucagon is a ligand for a specific G-protein linked receptor whose signalling pathway controls cell proliferation.
Two of the other peptides are secreted from gut endocrine cells and promote nutrient absorption through distinct mechanisms.
Finally, the fourth peptide is similar to glicentin, an active enteroglucagon.
Applications:Suitable for use in ELISA, Western Blot, Flow Cytometry (Not Tested), Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:ELISA: 1:1000Western Blot: 1:50-1:100Flow Cytometry (Not Tested): 1:10-1:50Immunofluorescence: 1:10-1:50Immunohistochemistry (FFPE): 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
Aliquots are stable at -20°C for 12 months after receipt.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Specificity：Recognizes human Glucagon.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：Synthetic peptide corresponding to a portion of amino acids within aa126-156 at the C-terminal region of human Glucagon, conjugated to KLH (P01275).
