84-3474-29　［取扱停止］Isocitrate Dehydrogenase, CT (Isocitrate dehydrogenase [NAD] subunit alpha, mitochondrial [Precursor], Isocitric dehydrogenase, NAD(+)-specific ICDH, IDH3A, IDH3) (Biotin) 200ul　I8850-01E-Biotin

特徴

• Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate.
• These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+).
• Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic.
• NAD(+)-dependent isocitrate dehydrogenases catalyze the allosterically regulated rate-limiting step of the tricarboxylic acid cycle.
• Each isozyme is a heterotetramer that is composed of two alpha subunits, one beta subunit, and one gamma subunit.
• The protein described here is the alpha subunit of one isozyme of NAD(+)-dependent isocitrate dehydrogenase.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000 Western Blot: 1:100-1:500Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.