84-3473-76　［取扱停止］PRMT7, CT (Protein Arginine N-methyltransferase 7, PRMT-7, Histone-arginine N-methyltransferase PRMT7, [Myelin Basic Protein]-arginine N-methyltransferase PRMT7, KIAA1933) (Biotin) 200ul　P9004-35B-Biotin

特徴

• Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity.
• At least three types of PRMT enzymes have been identified in mammalian cells.
• These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas.
• These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins.
• The mammalian PRMT family currently consists of 7 members that share two large domains of homology.
• Outside of these domains, epitopes were identified and antibodies against all 7 PRMT members have been developed.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C if to be used immediately within two weeks.
• For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20°C.
• Aliquots are stable at -20°C for 12 months after receipt.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Note: Applications are based on unconjugated antibody.