Retrograde golgi transport homolog 1 (RGP1) is the mammalian homolog to the yeast RGP1, and is thought to be a potential Golgi recycling factor.
The protein forms a tight complex with RIC1 and this complex binds Ypt6p and stimulates guanine nucleotide exchange.
RGP1 yeast mutants exhibit defects in retrograde trafficking that are similar to those seen in yeast, with mutations in other retrograde Golgi transport proteins.
It is expected that RGP1 plays a similar role in mammalian cells.
Applications:Suitable for use in ELISA and Western Blot.
Other applications not tested.
Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C in the dark.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
Caution: APC conjugates are sensitive to light.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide and a stabilizer. Labeled with Allophycocyanin (APC).
Specificity:Recognizes human RGP1. Species Crossreactivity: mouse.
Isotype:IgG
Calc Applications Abbrev:E WB
Calc Crossreactivity:Hu Mo
Immunogen:Synthetic peptide selected from the C-terminal region of human RGP1 (KLH).