84-3454-99　［取扱停止］LSD1, NT (Lysine-specific Histone Demethylase 1A, Amine Oxidase Flavin Containing Domain Protein 2, BRAF35-HDAC Complex Protein BHC110, AOF2, KIAA0601, KDM1) (APC) 200ul　L4595-50N-APC

特徴

• LSD1 is a histone demethylase that specifically demethylates 'Lys-4' of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor.
• LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain.
• This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase.
• It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed.
• LSD1 demethylates both mono- and tri-methylted 'Lys-4' of histone H3.
• This protein may play a role in the repression of neuronal genes.
• Alone, it is unable to demethylate H3 'Lys-4' on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity.
• It may also demethylate 'Lys-9' of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.
• Applications:Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:100-1:500Immunohistochemistry: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C in the dark.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• Caution: APC conjugates are sensitive to light.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.