84-3454-22　［取扱停止］UBE2N (Ubiquitin-conjugating Enzyme E2 N, Bendless-like Ubiquitin-conjugating Enzyme, Ubc13, UbcH13, Ubiquitin carrier Protein N, Ubiquitin-Protein Ligase N, UBE2N, BLU) (AP) 200ul　302824-AP

特徴

• The UBE2V1-UBE2N and UBE2V2-UBE2N heterodimers catalyze the synthesis of non-canonical 'Lys-63'-linked polyubiquitin chains.
• This type of polyubiquitination does not lead to protein degradation by the proteasome.
• Mediates transcriptional activation of target genes.
• Plays a role in the control of progress through the cell cycle and differentiation.
• Plays a role in the error-free DNA repair pathway and contributes to the survival of cells after DNA damage.
• Acts together with the E3 ligases, HLTF and SHPRH, in the 'Lys-63'-linked poly-ubiquitination of PCNA upon genotoxic stress, which is required for DNA repair.
• Appears to act together with E3 ligase RNF5 in the 'Lys-63'-linked polyubiquitination of JKAMP thereby regulating JKAMP function by decreasing its association with components of the proteasome and ERAD.
• Promotes TRIM5 capsid-specific restriction activity and the UBE2V1-UBE2N heterodimer acts in concert with TRIM5 to generate 'Lys-63'-linked polyubiquitin chains which activate the MAP3K7/TAK1 complex which in turn results in the induction and expression of NF-kappa-B and MAPK-responsive inflammatory genes (By similarity).
• Applications:Suitable for use in Western Blot.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.