84-3451-05　［取扱停止］HtrA3 (Serine Protease HTRA3, 3421-, High-temperature requirement Factor A3, Pregnancy-Related Serine Protease, HTRA3, PRSP) (AP) 200ul　302494-AP

特徴

• Insulin-like growth factors (IGFs) stimulate the proliferation and differentiation of a vast number of cell types.
• The action of the growth factors is mediated and controlled by a complex system of components, including several proteases that cleave the IGF-Binding Proteins.
• HtrA1 is a 480 aa protein that contains an N-terminus homologous to MAC25 (IGFBP7) with a conserved Kazal-type serine protease inhibitor motif, as well as a C-terminal PDZ domain.
• Semiquantitative RT-PCR and immunoblot analyses showed an approximately 7-fold increase of PRSS11 in osteoarthritis cartilage compared with controls.
• HTRA2 is released from mitochondria and inhibits the function of XIAP by direct binding in a way similar to SMAC.
• Moreover, when overexpressed extramitochondrially, HTRA2 induced atypical cell death, which was neither accompanied by a significant increase in caspase activity nor inhibited by caspase inhibitors, including XIAP.
• A catalytically inactive mutant of HTRA2, however, did not induce cell death.
• Suzuki et al.
• (2001) concluded that HTRA2 is a SMAC-like inhibitor of IAP (inhibitor of apoptosis proteins) activity with a serine protease-dependent cell death-inducing activity.
• Applications:Suitable for use in Western Blot, Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 1:1000Immunohistochemistry(Paraffin): 1:50~100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.