Ser/Thr-kinase component of cyclin D-CDK4 (DC) complexes that phosphorylate and inhibit members of the retinoblastoma (RB) protein family including RB1 and regulate the cell-cycle during G(1)/S transition.
Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complexes and the subsequent transcription of E2F target genes which are responsible for the progression through the G(1) phase.
Hypophosphorylates RB1 in early G(1) phase.
Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals.
Also phosphorylates SMAD3 in a cell-cycle-dependent manner and represses its transcriptional activity.
Component of the ternary complex, cyclin D/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex (By similarity).
Applications:Suitable for use in Immunofluorescence, Western Blot, Immunohistochemistry.
Other applications not tested.
Recommended Dilution:Immunohistochemistry(Paraffin): 1:25Immunofluorescence: 1:25Western Blot: 1:1000Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：100ul
Host：rabbit
Source Antibody：rat
Grade：Affinity Purified
Purity：Purified by Protein A affinity chromatography.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes rat R Cdk4. Species Crossreactivity: rat
Isotype：IgG
Calc Applications Abbrev：IF IHC WB
Calc Crossreactivity：Rt
Immunogen：KLH conjugated synthetic peptide corresponding to aa272-303 at the C-terminal region of rat Cdk4.
