Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation.
Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).
APG7 functions as an E1 enzyme essential for multisubstrates such as GABARAPL1 and ATG12.
APG3L is an E2-like conjugating enzyme facilitating covalent binding of APG8 (MAP1LC3) to phosphatidylethanolamine (PE).
APG7 (an E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with APG3.
Formation of the PE conjugate is essential for autophagy.
Applications:Suitable for use in Western Blot, Immunohistochemistry, ELISA
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Human, mouse
Isotype：IgG
Calc Applications Abbrev：E IHC WB
Calc Crossreactivity：Hu Mo
Immunogen：ATG7 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 291~321 amino acids from the Center of human APG7L.
