84-3442-34 [取扱停止]Hypoxia Inducible Factor 1 alpha, CT (Hypoxia-inducible Factor 1-alpha, HIF1-alpha, HIF-1alpha, HIF-1-alpha, HIF1A, HIF1, ARNT-interacting Protein, Basic-helix-loop-helix-PAS Protein MOP1, Class E Basic Helix-loop-helix Protein 78, bHLHe78, Member of PAS Protein 1, MOP1, PAS Domain-containing Protein 8, PASD8) (AP) 200ul H9804-03C-AP
84-3442-34 [取扱停止]Hypoxia Inducible Factor 1 alpha, CT (Hypoxia-inducible Factor 1-alpha, HIF1-alpha, HIF-1alpha, HIF-1-alpha, HIF1A, HIF1, ARNT-interacting Protein, Basic-helix-loop-helix-PAS Protein MOP1, Class E Basic Helix-loop-helix Protein 78, bHLHe78, Member of PAS Protein 1, MOP1, PAS Domain-containing Protein 8, PASD8) (AP) 200ul H9804-03C-AP
Hypoxia-inducible factor-1 (HIF1) is a transcription factor found in mammalian cells cultured under reduced oxygen tension that plays an essential role in cellular and systemic homeostatic responses to hypoxia.
HIF1 is a heterodimer composed of an alpha subunit and a beta subunit.
The beta subunit has been identified as the aryl hydrocarbon receptor nuclear translocator (ARNT).
Applications:Suitable for use in Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Western Blot: 1:100-1:500Immunofluorescence: 1:10-1:50Immunohistochemistry (FFPE): 1:10-1:50Optimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size:200ul
Host:rabbit
Source Antibody:human
Grade:Purified
Purity:Purified by ammonium sulfate precipitation.
Form:Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity:Recognizes human HIF1A at ~92kD. Species Crossreactivity: mouse
Isotype:IgG
Calc Applications Abbrev:IF IHC WB
Calc Crossreactivity:Hu Mo
Immunogen:KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids aa736-765 in the C-terminal region of human HIF1A, UniProt Accession #NP_001521; Q16665 Cellular Localization: Cytoplasm, Nucleus