84-3440-47　［取扱停止］CTSF, ID (D276) (Cathepsin F, CATSF) (AP) 200ul　C2097-42B-AP

特徴

• Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system.
• Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region.
• The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold.
• The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily.
• This cystatin-like domain contains some of the elements known to be important for inhibitory activity.
• CTSF is a predicted protein of 484 amino acids which contains a 19 residue signal peptide.
• Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway.
• Applications:Suitable for use in ELISA and Western Blot.
• Other applications not tested.
• Recommended Dilution:ELISA: 1:1,000Western Blot: 1:50-1:100Optimal dilutions to be determined by the researcher.
• Storage and Stability:Store product at 4°C.
• DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
• Dilute required amount only prior to immediate use.
• Further dilutions can be made in assay buffer.
• For maximum recovery of product, centrifuge the original vial prior to removing the cap.
• Note: Applications are based on unconjugated antibody.