PCNA is found in the nucleus and is a cofactor of DNA polymerase delta.
This protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication.
In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway.
Applications:Suitable for use in ELISA, Western Blot, Immunofluorescence and Immunohistochemistry.
Other applications not tested.
Recommended Dilutions:Immunohistochemistry: paraffinOptimal dilutions to be determined by the researcher.
Storage and Stability:Store product at 4°C.
DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid.
Dilute required amount only prior to immediate use.
Further dilutions can be made in assay buffer.
For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Note: Applications are based on unconjugated antibody.
仕様
Size：200ul
Host：rabbit
Source Antibody：human
Grade：Purified
Purity：Purified by ammonium sulfate precipitation.
Form：Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Specificity：Recognizes human PCNA.
Isotype：IgG
Calc Applications Abbrev：E IF IHC WB
Calc Crossreactivity：Hu
Immunogen：KLH-conjugated synthetic peptide mapping to a fragment of residues within 231-261 amino acids from the C-terminal region of human PCNA. Uniprot/Swiss Accession: P12004. Species sequence homology: bovine, hamster, monkey and rat
