84-3435-87　ZO1 (TJP1, Tight Junction Protein 1, Tight Junction Protein ZO-1, Tight Junction Protein ZO1, Tjp1, Zo-1, Zo1, ZO1_HUMAN, Zona Occludens 1, Zona Occludens 1 Protein, Zona Occludens Protein 1, Zonula Occludens 1 Protein, Zonula Occludens Protein 1) 100ug　303709

特徴

• Tight junction protein ZO-1 is a protein that in humans is encoded by the TJP1 gene.
• It is mapped to 15q13.1.
• This gene encodes a protein located on a cytoplasmic membrane surface of intercellular tight junctions.
• The encoded protein may be involved in signal transduction at cell–cell junctions.
• It has been found that injected CagA associates with the epithelial tight-junction scaffolding protein TJP1 and the transmembrane protein junctional adhesion molecule, causing an ectopic assembly of tight junction components at sites of bacterial attachment, and altering the composition and function of the apical-junctional complex.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.