84-3435-85　YBX1, CT (YB1, BP 8, CBF-A, CCAAT Binding Transcription Factor I Subunit A, CCAAT-binding Transcription Factor I Subunit A, CSDA2, CSDB, DBPB, DNA Binding Protein B, DNA-binding Protein B, EFI-A, Enhancer Factor I Subunit A, MDR NF1, NSEP 1, NSEP1, Nuclease Sensitive Element Binding Protein 1, Nuclease-sensitive Element-binding Protein 1, p50, Y-box Binding Protein 1, Y-box Transcription Facto ...　303707

特徴

• YBX1 (Y box binding protein 1), commonly referred to as "YB-1" by researchers, is a human protein.
• YB1 binding has an absolute requirement for the CCAAT box and relative specificity for the Y box.
• It has a molecular mass of 35,414 and contains 18% basic residues and putative nuclear localization signals.
• The YBX1 gene is mapped on 1p34.2.
• Ybx1 was highly expressed in mouse erythroid myeloid lymphoid clone-1 (EML), a hematopoietic precursor cell line, but that it was downregulated in myeloid progenitors and in Gmcsf-treated EML cells during myeloid differentiation.
• Ybx1 was expressed at high levels in myeloid leukemic cells at different developmental stages.
• Knockdown of YBX1 in a human leukemic cell line inhibited proliferation ability, induced apoptosis, and induced megakaryocytic differentiation in response to arsenic trioxide treatment.
• YBX1 is downregulated during myeloid differentiation and aberrant YBX1 expression in leukemic cells may contribute to leukemia development by blocking differentiation.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (frozen): 0.5-1ug/mlImmunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.