84-3435-84　XRCC5, CT (Ku80, 86kD Subunit of Ku Antigen, ATP Dependent DNA Helicase 2 Subunit 2, ATP Dependent DNA Helicase II 80kD Subunit, ATP Dependent DNA Helicase II 86Kd Subunit, ATP Dependent DNA Helicase II, ATP-dependent DNA Helicase 2 Subunit 2, ATP-dependent DNA Helicase II 80kD Subunit, CTC Box Binding Factor 85kD, CTC Box-binding Factor 85kD Subunit, CTC85, CTCBF, DNA Repair Protein XRCC5, KA ...　303706

特徴

• XRCC5 (X-ray Repair, Complementing Defective , In Chinese Hamster, 5), also known as Ku80 or Ku86, is a protein that in humans, is encoded by the XRCC5 gene.
• The XRCC5 gene encodes the 80kD subunit of the Ku autoantigen, a heterodimer which contributes to genomic integrity through its ability to bind DNA double-strand breaks and facilitate repair by the nonhomologous end joining (NHEJ) pathway.
• The XRCC5 gene is mapped to 2q35.
• Human colon cancer cells heterozygous for Ku86 are haplo insufficient with an increase in polyploid cells, a reduction in cell proliferation, elevated p53 levels, and a slight hypersensitivity to ionizing radiation.
• Functional inactivation of the second Ku86 allele results in cells with a drastically reduced doubling time.
• The Ku86 locus is essential in human somatic tissue culture cells by experiments demonstration.
• A rare microsatellite polymorphism in XRCC5 is associated with cancer in patients of varying radiosensitivity.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.