84-3435-83　XBP1, NT (Tax Responsive Element Binding Protein 5, Tax-responsive Element-binding Protein 5, TREB5, X Box Binding Protein 1, X Box Binding Protein 2, X-box-binding Protein 1, XBP 1, XBP-1, XBP1_HUMAN, XBP2) 100ug　303705

特徴

• XBP1, also known as X-box binding protein 1, is a protein which in humans is encoded by the XBP1 gene.
• It encodes a transcription factor that regulates MHC class II genes by binding to a promoter element referred to as an X box.
• This gene product is a bZIP protein, which was also identified as a cellular transcription factor that binds to an enhancer in the promoter of the T cell leukemia virus type 1 promoter.
• It may increase expression of viral proteins by acting as the DNA binding partner of a viral transactivator.
• It has been found that upon accumulation of unfolded proteins in the endoplasmic reticulum (ER), the mRNA of this gene is processed to an active form by an unconventional splicing mechanism that is mediated by the endonuclease inositol-requiring enzyme 1 (IRE1).
• The resulting loss of 26 nt from the spliced mRNA causes a frame-shift and an isoform XBP1(S), which is the functionally active transcription factor.
• The isoform encoded by the unspliced mRNA, XBP1(U), is constitutively expressed, and thought to function as a negative feedback regulator of XBP1(S), which shuts off transcription of target genes during the recovery phase of ER stress.
• A pseudogene of XBP1 has been identified and localized to chromosome 5.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.1ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.