84-3435-82　Wnt5A (HWNT 5A, HWNT5A, Protein Wnt 5a, Protein Wnt-5a, Protein Wnt5a, Wingless Type MMTV Integration Site Family Member 5A, Wnt 5a, WNT 5A Protein, WNT 5A Protein Precursor, WNT5A, WNT5A Protein Precursor) 100ug　303704

特徴

• Protein Wnt-5a is a protein that in humans is encoded by the WNT5A gene.
• This gene is a member of the WNT gene family.
• The WNTs comprise a large class of secreted proteins that control essential developmental processes such as embryonic patterning, cell growth, migration, and differentiation.
• It is mapped to 3p14.3.
• WNT5A is highly expressed in the dermal papilla of depilated skin.
• This protein plays an essential role in regulating developmental pathways during embryogenesis.
• And this protein may also play a role in oncogenesis.
• WNT5A and DVL can promote axon differentiation mediated by the PAR3-PAR6-aPKC complex.
• Applications:Suitable for use in Western Blot and Immunohistochemistry.
• Other applications not tested.
• Recommended Dilution:Western Blot: 0.1-0.5ug/ml, the detection limit is ~0.25ng/lane under reducing conditions
• Immunohistochemistry (paraffin): 0.5-1ug/ml, boiling the paraffin sections in 10mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections
• Optimal dilutions to be determined by the researcher.
• Storage and Stability:Lyophilized powder may be stored at -20°C.
• Stable for 12 months after receipt at -20°C.
• Reconstitute with sterile ddH2O.
• Aliquot to avoid repeated freezing and thawing.
• Store at -20°C.
• Reconstituted product is stable for 12 months at -20°C.
• For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
• Further dilutions can be made in assay buffer.